Polarised Migration: Cofilin Holds the Front

Polarised Migration: Cofilin Holds the Front

Persistent cell locomotion is a key feature of eukaryotic cells responding to diverse physiological cues. New work now directly implicates ADF/cofilin proteins as essential regulators of polarised cell migration.

ADF/Cofilin Controls Cell Polarity during Fibroblast Migration

To migrate, normally a cell must establish morphological polarity and continuously protrude a single lamellipodium, polarized in the direction of migration. We have previously shown that actin filament disassembly is necessary for protrusion of the lamellipodium during fibroblast migration. As ADF/cofilin (AC) proteins are essential for the catalysis of filament disassembly in cells, we assesse...

Who holds the leash?

Downregulation of LIMK1–ADF/cofilin by DADS inhibits the migration and invasion of colon cancer

This study aimed to explore whether the downregulation of LIM kinase 1 (LIMK1)-actin depolymerization factor (ADF, also known as destrin)/cofilin by diallyl disulfide (DADS) inhibited the migration and invasion of colon cancer. Previous studies have shown that silencing LIMK1 could significantly enhance the inhibitory effect of DADS on colon cancer cell migration and invasion, suggesting that L...

Cofilin takes the lead.

Cofilin has emerged as a key regulator of actin dynamics at the leading edge of motile cells. Through its actin-severing activity, it creates new actin barbed ends for polymerization and also depolymerizes old actin filaments. Its function is tightly regulated in the cell. Spatially, its activity is restricted by other actin-binding proteins, such as tropomyosin, which compete for accessibility...